Inline PAT for Aggregating Cells

Real-time aggregate monitoring for cell manufacturing.

Inline machine-vision analytics for continuous monitoring of aggregate size, morphology, and formation dynamics. Side-mounted, closed-system autosampling from any vessel format, without manual intervention.

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The Morphokinetics PAT Platform

Most suspension cell cultures are still monitored blind.

The majority of cell manufacturing teams still monitor their suspension cultures by pulling manual samples once or twice a day. Each sample is invasive, introduces contamination risk, and captures only a single snapshot of a continuously changing process.

Between those samples, critical changes in aggregate size and morphology go entirely unobserved. By the time a deviation is detected, the process has already drifted, impacting consistency, yield, and downstream performance.

From snapshots to continuous insight

Manual sampling: 1-2 invasive data points per day. You are flying blind between samples, with contamination risk at every pull.

Morphokinetics: Closed-system autosampling up to every 15 minutes. Continuous, non-invasive, from any vessel format. You see the full picture.

Aggregate formation is dynamic, not static.

Size and morphology shift continuously in response to shear conditions, growth kinetics, and changes in media composition or handling. These are not step changes you can catch with a daily sample. They are gradual trajectories that only become visible with continuous observation.

Snapshot measurements miss these dynamics entirely. A culture that looks acceptable at the morning sample may already be drifting out of specification by the time you check again.

Aggregate segmentation, identification, and size distribution analysis

Quantitative, not subjective

Our machine vision algorithms automatically detect, segment, and measure every aggregate in each frame. Raw brightfield captures are processed into per-aggregate measurements including equivalent diameter, morphology descriptors, and population-level size distributions.

The result: objective, statistical data on your aggregate population at every time point, replacing manual assessment with continuous quantitative insight.

Every run builds a high-resolution dataset of aggregate formation dynamics, creating the foundation for predictive process models.

When you can see the process, you can control it.

Suspension cultures are notoriously variable batch to batch. With continuous visibility into aggregate behaviour, drift is flagged as it emerges, not hours later, giving you the chance to path-correct before it costs you the run. Aggregate size becomes a controllable parameter rather than a measured output, particularly in iPSC expansion where it must be held tightly over time.

Real-time intervention becomes possible:

Adjust agitation and shear conditions before aggregates move out of specification

Modify feeding strategies in response to observed formation kinetics

Stabilise aggregate size distributions before they impact downstream performance

Identify failing batches early enough to rescue or abandon before wasting further resources

We built this system from idea to working prototype in six months, combining cell biology, machine vision, and hardware engineering under one roof. That is how we work.

From intermittent sampling to continuous process visibility.

The Morphokinetics PAT platform provides inline, closed-system monitoring of aggregate dynamics inside your culture. No manual sampling, no contamination risk. Currently in active customer evaluation.

MorphoKinetics PAT system mounted on a benchtop incubator in a Cambridge cell culture lab

Mounting

Side-mounted on the incubator or bioreactor. No modification to your existing vessel or protocol, and no loss of sample. Compatible with iPSC, MSC, and organoid suspension cultures. Patent-pending hardware.

Sampling

Automated closed-system autosampling at up to 15-minute intervals across the entire culture run.

Compatibility

Shake flasks through to stirred-tank bioreactors. Designed for integration with industry-standard vessel formats and SCADA stacks.

What you get

Aggregate size distribution, morphology classification, and formation kinetics at every time point.

The cell therapy industry is shifting from manual, batch-driven manufacturing toward automated, data-rich, and continuously monitored production. Morphokinetics is built for this transition. Our PAT platform provides the real-time process data that closed-loop control, digital twins, and adaptive manufacturing strategies require.

iPSCs MSCs iPSC-Derived Organoids Primary Tissue Organoids

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Our Team

Built by people who have lived the problem.

Morphokinetics was founded by scientists and engineers with backgrounds spanning electronics engineering, bioprocess and cell manufacturing, and iPSC gene engineering and differentiation for cell therapy. We built the company to solve the problems we kept running into ourselves.

Get in Touch

Let's talk.

Whether you are interested in beta access to our PAT platform or exploring vessel integration, we would like to hear from you.

BioInnovation Centre, Building 25
Cambridge Science Park Road
Milton, Cambridge, CB4 0FW
United Kingdom

info@morphokinetics.com